Open AccessChemical mapping of co-existing RNA structures
Author(s) -
A. R. W. Schröder,
Tilman Baumstark,
Detlev Riesner
Publication year - 1998
Publication title -
nucleic acids research
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/26.14.3449
Subject(s) - biology , primer extension , rna , nucleic acid structure , gel electrophoresis , computational biology , primer (cosmetics) , electrophoresis , biochemistry , gene , chemistry , organic chemistry
In many cases RNA can assume co-existing or meta-stable structures preventing structure determination by chemical mapping. A novel method is described, by which RNA is modified with dimethyl sulphate without shifting the distribution of different structures. The different structures are then separated in native gel electrophoresis, and structure determination by primer extension can be carried out separately for each structure.
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