Rapid isolation of yeast plasmids as native chromatin | Zendy

Carlos MartínezCampa | Zendy; Nicholas A. Kent | Zendy; Jane Mellor | Zendy

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Rapid isolation of yeast plasmids as native chromatin

Author(s) -

Carlos MartínezCampa,

Nicholas A. Kent,

Jane Mellor

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.9.1872

Subject(s) - chromatin , biology , plasmid , chia pet , yeast , genetics , dna , microbiology and biotechnology , isolation (microbiology) , chromatin remodeling , bioinformatics

Many regions of chromatin are subject to dynamic changes. We have developed a rapid method for isolation of small chromatin templates from yeast which will facilitate biochemical analysis of chromatin composition. Using the PHO5 promoter we show that templates prepared from cells grown in inducing or repressing conditions show native chromatin structures. This method may be widely applicable as the chromatin structures at a centromere, at ARS1 and at part of the lacZ region on two other plasmids are preserved after chromatin isolation.

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