B lymphocyte-specific, Cre-mediated mutagenesis in mice | Zendy

Regan Rickert | Zendy

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B lymphocyte-specific, Cre-mediated mutagenesis in mice

Author(s) -

Regan Rickert

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.6.1317

Subject(s) - biology , cd19 , cre lox recombination , microbiology and biotechnology , mutagenesis , gene , gene targeting , cre recombinase , bone marrow , genetics , mutation , transgene , immunology , genetically modified mouse , flow cytometry

Adaptation of the P1 phage-derived Cre /loxP site- specific recombination system to the gene targeting technique allows for the conditional deletion of genes in mice. To selectively modify genes in B lymphocytes, we have generated mice (designated CD19-Cre) which express cre under the transcriptional control of the B lineage-restricted CD19 gene. In a model system involving the cross of CD19-Cre mice with mice bearing a loxP -flanked substrate, we find a deletion efficiency of 75-80% in bone marrow-derived pre-B cells that increases to 90-95% in splenic B cells.

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