Open AccessColorimetric detection of immobilised PCR products generated on a solid support
Author(s) -
Andrew K. Lockley
Publication year - 1997
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/25.6.1313
Subject(s) - biology , digoxigenin , microbiology and biotechnology , detection limit , computational biology , chromatography , biochemistry , gene , gene expression , chemistry , in situ hybridization
By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.
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