Open AccessMammalian cDNA and Prokaryotic Reporter Sequences Silence Adjacent Transgenes in Transgenic Mice
Author(s) -
Anthony Clark,
Graham Harold,
Fiona E. Yull
Publication year - 1997
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/25.5.1009
Subject(s) - biology , complementary dna , reporter gene , transgene , gene , microbiology and biotechnology , gene silencing , genetically modified mouse , gene expression , genetics
The ovine beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a second gene construct comprising a mammalian cDNA or a CAT reporter sequence it fails to be expressed in the majority of transgenic lines generated. We suggest that mammalian cDNAs and prokaryotic reporter sequences can serve as active foci for gene silencing in the mammalian genome.
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