Simultaneous display of different peptides on the surface of filamentous bacteriophage | Zendy

Pratap Malik | Zendy; Richard N. Perham | Zendy

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Simultaneous display of different peptides on the surface of filamentous bacteriophage

Author(s) -

Pratap Malik,

Richard N. Perham

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.4.915

Subject(s) - biology , bacteriophage , filamentous bacteriophage , plasmid , gene , phage display , computational biology , genetics , peptide , escherichia coli , biochemistry

We have developed a new system for producing hybrid virions of filamentous bacteriophage fd simultaneously displaying two different peptides by infecting cells harbouring a plasmid containing a modified gene VIII with an engineered bacteriophage carrying a second and different copy of a modified gene VIII. The simultaneous display of different peptides has many potential applications in exploring the immune response and studying protein-protein interaction.

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