DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter | Zendy

Didier Négre | Zendy; Christelle BonodBidaud | Zendy; C. Oudot | Zendy; Jf Prost | Zendy; A. Kolb | Zendy; A. Ishihama | Zendy; Aj Cozzone | Zendy; Jc Cortay | Zendy

Open Access

DNA flexibility of the UP element is a major determinant for transcriptional activation at the Escherichia coli acetate promoter

Author(s) -

Didier Négre,

Christelle BonodBidaud,

C. Oudot,

Jf Prost,

A. Kolb,

A. Ishihama,

Aj Cozzone,

Jc Cortay

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.4.713

Subject(s) - biology , operon , rna polymerase , microbiology and biotechnology , sigma factor , escherichia coli , promoter , base pair , specificity factor , rna polymerase i , polymerase , rna , dna , biochemistry , gene , gene expression

The specific interaction of the upstream element-containing promoter of the Escherichia coli acetate operon with either the RNA polymerase holoenzyme or its alpha subunit has been analyzed by the base removal method. Our results indicate that: (i) direct and specific base contacts can be detected in the acetate promoter-alpha subunit complex; (ii) base elimination in the upstream element of the acetate promoter enhances the binding of RNA polymerase. A similar effect is observed when studying the interactions between RNA polymerase and the rrnB ribosomal operon P1 promoter.

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