Betaine Improves the PCR Amplification of GC-Rich DNA Sequences | Zendy

Wolfgang Henke | Zendy; K. Herdel | Zendy; Klaus Jung | Zendy; Dietmar Schnorr | Zendy; S.A. Loening | Zendy

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Betaine Improves the PCR Amplification of GC-Rich DNA Sequences

Author(s) -

Wolfgang Henke,

K. Herdel,

Klaus Jung,

Dietmar Schnorr,

S.A. Loening

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.19.3957

Subject(s) - biology , betaine , complementary dna , multiple displacement amplification , microbiology and biotechnology , dna , gc content , polymerase chain reaction , genetics , gene , genome , biochemistry , dna extraction

Betaine improves the co-amplification of the two alternatively spliced variants of the prostate-specific membrane antigen mRNA as well as the amplification of the coding cDNA region of c-jun. It is suggested that betaine improves the amplification of these genes by reducing the formation of secondary structure caused by GC-rich regions and, therefore, may be generally applicable to ameliorate the amplification of GC-rich DNA sequences.

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