Open AccessA Rapid Method for Chromatin Structure Analysis in the Filamentous Fungus Aspergillus Nidulans
Author(s) -
Ramón González,
Claudio Scazzocchio
Publication year - 1997
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/25.19.3955
Subject(s) - aspergillus nidulans , biology , chromatin , filamentous fungus , nucleosome , protoplast , nuclease , genetics , microbiology and biotechnology , dna , gene , mutant
A rapid method for nuclease digestion of Aspergillus nidulans chromatin is described. It overcomes the need for nuclear purification or protoplast preparation. The method is valid for the analysis of the nucleosomal repeat length in bulk chromatin, and allows the analysis of nucleosome phasing at a specific locus.
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