Improved efficiency sos recruitment system: expression of the mammalian GAP reduces isolation of Ras GTPase false positives | Zendy

Ami Aronheim | Zendy

Open Access

Improved efficiency sos recruitment system: expression of the mammalian GAP reduces isolation of Ras GTPase false positives

Author(s) -

Ami Aronheim

Publication year - 1997

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/25.16.3373

Subject(s) - biology , false positive paradox , gtpase activating protein , gtpase , guanine nucleotide exchange factor , microbiology and biotechnology , fusion protein , small gtpase , complementary dna , isolation (microbiology) , computational biology , genetics , bioinformatics , gene , signal transduction , recombinant dna , g protein , machine learning , computer science

The Sos recruitment system (SRS) is a novel genetic method for detecting protein-protein interactions. The method is based on localizing Sos, a Ras guanyl nucleotide exchange factor (GEF), to the plasma membrane through interaction between two fusion proteins. Mammalian Ras can bypass the requirement for a functional Ras GEF and represents a predictable false positive in this system. This report demonstrates that introduction of mammalian GTPase activating protein (mGAP) reduces the isolation of Ras false positives in SRS screens of mammalian cDNA libraries, thereby significantly enhancing the efficiency of the system.

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