The Polypyrimidine Tract Binding (PTB) Protein Interacts With Single-Stranded DNA in a Sequence-Specific Manner | Zendy

Françoise Brunel | Zendy; Mario M. Zakin | Zendy; H. Buc | Zendy; Malcolm Buckle | Zendy

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The Polypyrimidine Tract Binding (PTB) Protein Interacts With Single-Stranded DNA in a Sequence-Specific Manner

Author(s) -

Françoise Brunel,

Mario M. Zakin,

H. Buc,

Malcolm Buckle

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.9.1608

Subject(s) - polypyrimidine tract binding protein , biology , dna , dna binding protein , microbiology and biotechnology , electrophoretic mobility shift assay , polypyrimidine tract , sequence (biology) , rna , rna binding protein , genetics , transcription factor , gene

Polypyrimidine tract binding (PTB) protein is a cellular factor whose function is unknown. Various RNA or single-stranded DNA sequences have been shown to interact with PTB. In this paper, using laser UV crosslinking and electrophoretic mobility shift assays to probe DNA-protein interactions, we demonstrate that PTB binding at a single-stranded DNA target is highly sequence-specific. We provide data showing that PTB interacts with the top strand of the adenovirus major late promoter transcriptional initiator, a sequence rich in pyrimidine residues. We also demonstrate that PTB is organised into at least two different binding domains.

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