Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication | Zendy

Lothar Halmer | Zendy

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Effects of cell cycle dependent histone H1 phosphorylation on chromatin structure and chromatin replication

Author(s) -

Lothar Halmer

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.8.1420

Subject(s) - histone h1 , biology , chromatin , histone h2a , histone , histone octamer , microbiology and biotechnology , histone code , nucleosome , genetics , dna

We have reconstituted salt-treated SV40 minichromosomes with differentially phosphorylated forms of histone H1 extracted from either G0-, S- or M-phase cells. Sedimentation studies revealed a clear difference between minichromosomes reconstituted with S-phase histone H1 compared with histone H1 from G0- or M-phase cells, indicating that the phosphorylation state of histone H1 has a direct effect on chromatin structure. Using reconstituted minichromosomes as substrate in the SV40 in vitro replication system, we measured a higher replication efficiency for SV40 minichromosomes reconstituted with S-phase histone H1 compared with G0- or M-phase histone H1. These data indicate that the chromatin structure induced by the phosphorylation of histone H1 influences the replication efficiency of SV40 minichromosomes in vitro.

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