Construction of a Chromosome Specific Library of Human Mars and Mapping of Matrix Attachment Regions on Human Chromosome 19 | Zendy

Л. Г. Николаев | Zendy; T. Tsevegiyn | Zendy; С. Б. Акопов | Zendy; Linda K. Ashworth | Zendy; Е. Д. Свердлов | Zendy

Open Access

Construction of a Chromosome Specific Library of Human Mars and Mapping of Matrix Attachment Regions on Human Chromosome 19

Author(s) -

Л. Г. Николаев,

T. Tsevegiyn,

С. Б. Акопов,

Linda K. Ashworth,

Е. Д. Свердлов

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.7.1330

Subject(s) - biology , mars exploration program , chromosome , scaffold/matrix attachment region , genetics , matrix (chemical analysis) , gene mapping , computational biology , gene , astrobiology , gene expression , materials science , chromatin remodeling , composite material

Using a novel procedure a representative human chromosome 19-specific library was constructed of short sequences, which bind preferentially to the nuclear matrix (matrix attachment regions, or MARs). Judging by 20 clones sequenced so far, the library contains > 50% of human inserts, about 90% of which are matrix-binding by the in vitro test. Computer analysis of sequences of eight human MARs did not reveal any significant homologies with the EMBL Nucleotide Data Base entries as well as between MARs themselves. Eight MARs were assigned to individual positions on the chromosome 19 physical map. The library constructed can serve as a good source of MAR sequences for comparative analysis and classification and for further chromosome mapping of MARs as well.

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