Sequence Analysis of 56 Kb from the Genome of the Bacterium Mycoplasma Pneumoniae Comprising the dnaA Region, the Atp Operon and a Cluster of Ribosomal Protein Genes | Zendy

H. Hilbert | Zendy; Ralf Himmelreich | Zendy; H. Plagens | Zendy; Richard Herrmann | Zendy

Open Access

Sequence Analysis of 56 Kb from the Genome of the Bacterium Mycoplasma Pneumoniae Comprising the dnaA Region, the Atp Operon and a Cluster of Ribosomal Protein Genes

Author(s) -

H. Hilbert,

Ralf Himmelreich,

H. Plagens,

Richard Herrmann

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.4.628

Subject(s) - biology , operon , cosmid , plasmid , genetics , dnaa , ecori , gene , genome , ribosomal protein , ribosomal rna , genomic library , microbiology and biotechnology , escherichia coli , origin of replication , peptide sequence , rna , ribosome

To sequence the entire 800 kilobase pair genome of the bacterium Mycoplasma pneumoniae, a plasmid library was established with contained the majority of the EcoR1 fragments from M.pneumoniae. The EcoR1 fragments were subcloned from an ordered cosmid library comprising the complete M.pneumoniae genome. Individual plasmid clones were sequenced in an ordered fashion mainly by primer walking. We report here the initial results from the sequence analysis of -56 kb comprising the dnaA region as a potential origin of replication, the ATPase operon and a region coding for a cluster of ribosomal protein genes. The data were compared with the corresponding genes/operons from Bacillus subtilis, Escherichia coli, Mycoplasma capricolum and Mycoplasma gallisepticum.

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