Molecular haplotyping of genetic markers 10 kb apart by allele-specific long-range PCR | Zendy

S Michalatos-Beloin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Molecular haplotyping of genetic markers 10 kb apart by allele-specific long-range PCR

Author(s) -

S Michalatos-Beloin

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.23.4841

Subject(s) - biology , haplotype , genetics , allele , locus (genetics) , mendelian inheritance , genome , ploidy , alu element , allele frequency , gene , human genome

Haplotypes, combinations of polymorphic markers in a chromosome, are critical for genome diversity research. However, their utility in population samplings is compromised by uncertain linkage phase determinations from unrelated individuals. Molecular haplotyping accomplishes direct phase determination by generation of hemizygous templates from diploid genomic samples. We report molecular haplotyping by allele-specific long-range PCR of two markers 9.5 kb apart at the CD4 locus: a bi-allelic Alu deletion and a multi-allelic repeat. We verified CD4 molecular haplotypes by classical Mendelian analysis. Molecular haplotyping should prove useful in mapping disease genes and in establishing founder effects.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research