Synthesis of full-length oligonucleotides: cleavage of apurinic molecules on a novel support
Author(s) -
Marek Kwiatkowski
Publication year - 1996
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/24.23.4632
Subject(s) - depurination , oligonucleotide , biology , cleavage (geology) , molecule , dna , ap site , combinatorial chemistry , bond cleavage , chromomycin a3 , computational biology , biochemistry , dna damage , chemistry , chromatin , paleontology , organic chemistry , heterochromatin , fracture (geology) , catalysis
The synthesis of oligodeoxynucleotides is marred by several problems that contribute to the formation of defective molecules. This in turn seriously limits the usefulness of such reagents in DNA diagnostics, molecular cloning, DNA structural analysis and in antisense therapy. In particular, depurination reactions during the cyclical steps of synthesis lead to strand scission during cleavage of the completed molecules from the support. Here we present a remedy to this problem: a novel disiloxyl linkage that connects oligonucleotides to the support withstands reaction conditions that allow the removal of the 5' parts of any depurinated molecules. This ensures that all molecules that preserve the 5' protecting group when cleaved from the support will have both correct 3'- and 5'-ends. We demonstrate the application of the support for synthesis of padlock probe molecules.
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