A Cost Effective Base-Matching Assay with Low Backgrounds | Zendy

Xing Su | Zendy; G. Mushinsky | Zendy; Anne Marie Comeau | Zendy

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A Cost Effective Base-Matching Assay with Low Backgrounds

Author(s) -

Xing Su,

G. Mushinsky,

Anne Marie Comeau

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.22.4596

Subject(s) - biology , genotyping , computational biology , genetics , base (topology) , base sequence , base pair , matching (statistics) , mutation , dna sequencing , identification (biology) , oligonucleotide , genotype , dna , gene , statistics , mathematical analysis , botany , mathematics

Base-matching or so-called mini-sequencing is a powerful technique for genotyping and mutation identification. However, its application is often hampered by high background and high cost. We have decreased the background by approximately 5-fold by incorporating an end-blocking step and using only 1/10 of the usual nucleotide concentrations.

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