A method for linking yeast artificial chromosomes | Zendy

Zoia Larin | Zendy; Stephen S. Taylor | Zendy; Chris TylerSmith | Zendy

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A method for linking yeast artificial chromosomes

Author(s) -

Zoia Larin,

Stephen S. Taylor,

Chris TylerSmith

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.21.4192

Subject(s) - biology , yeast artificial chromosome , homologous recombination , homologous chromosome , genetics , yeast , vector (molecular biology) , gene , reporter gene , mitosis , human artificial chromosome , shuttle vector , sequence (biology) , plasmid , microbiology and biotechnology , computational biology , recombinant dna , chromosome , gene expression , gene mapping

A method for linking any standard yeast artificial chromosomes (YAC) is described. YACs are introduced into the same cell and joined by mitotic recombination between the vector arms and the homologous sequence in a linking vector; several YACs can be recombined sequentially. The linking vectors also contain the beta-galactosidase gene as an expression reporter in mammalian cells.

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