Improved immobilization of DNA to microwell plates for DNA-DNA hybridization | Zendy

Hisako Hirayama | Zendy; Jin Támaoka | Zendy; Koki Horikoshi | Zendy

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Improved immobilization of DNA to microwell plates for DNA-DNA hybridization

Author(s) -

Hisako Hirayama,

Jin Támaoka,

Koki Horikoshi

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.20.4098

Subject(s) - biology , dna , dna–dna hybridization , microbiology and biotechnology , sequencing by hybridization , nucleic acid thermodynamics , hybridization probe , dna sequencing , genetics , base sequence , dna sequencer

An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. This simple new protocol can reduce the consumption of scarce DNA samples.

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