Effect on DNA transcription of nucleotide sequences upstream to T7 promoter | Zendy

Michail M. Baklanov | Zendy; L. N. Golikova | Zendy; Enrst G. Malygin | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Effect on DNA transcription of nucleotide sequences upstream to T7 promoter

Author(s) -

Michail M. Baklanov,

L. N. Golikova,

Enrst G. Malygin

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.18.3659

Subject(s) - biology , transcription (linguistics) , upstream activating sequence , t7 rna polymerase , base pair , nucleic acid sequence , promoter , microbiology and biotechnology , genetics , dna , rna polymerase , consensus sequence , rna , gene , gene expression , base sequence , bacteriophage , escherichia coli , philosophy , linguistics

The T7 RNA polymerase-dependent transcription was studied as a function of nucleotide sequence structures positioned upstream of the T7 promoter. Model double-stranded DNA templates were constructed for this purpose. They contained a target sequence of 485 base pairs (cDNA fragment of Venesuelian encephalomyelitis equine virus genome), T7 promoter consensus and different extra base sequences upstream of the T7 promoter. The level of the target sequence transcription was clearly determined by the extra base sequence. The presence of one extra base pair G.C ensured the most pronounced effect, transcription was increased one order of magnitude in comparison with template which has only a canonical T7 promoter sequence at the 5'-end.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research