A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting | Zendy

Kazuto Kobayashi | Zendy

Open Access

A novel strategy for the negative selection in mouse embryonic stem cells operated with immunotoxin-mediated cell targeting

Author(s) -

Kazuto Kobayashi

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.18.3653

Subject(s) - immunotoxin , biology , embryonic stem cell , recombinant dna , stem cell , cytotoxic t cell , microbiology and biotechnology , negative selection , receptor , cell , cell culture , mutation , cancer research , genetics , antibody , monoclonal antibody , gene , in vitro , genome

Immunotoxoin-mediated cell targeting (IMCT) is a technique for conditionally ablating specific cell types based on the cytotoxic activity of a recombinant immunotoxin anti-Tac (Fv)-PE40. To examine the feasibility of this technique for the negative selection in mouse embryonic stem (ES) cells, we investigated the responsiveness of cells expressing human interleukin-2 receptor alpha subunit to anti-Tac(Fv)-PE40. The immunotoxin treatment efficiently eliminated only ES cells bearing the receptor as a consequence of the target specificity of anti-Tac(Fv)-PE40, indicating that IMCT can be used as a novel strategy for positive and negative selection to enrich ES cell clones with a targeted mutation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research