A rapid method for detecting specific amplified PCR fragments in microtiter plates | Zendy

Alma Ortiz | Zendy; E. Ritter | Zendy

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A rapid method for detecting specific amplified PCR fragments in microtiter plates

Author(s) -

Alma Ortiz,

E. Ritter

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.16.3280

Subject(s) - biology , oligonucleotide , microbiology and biotechnology , nucleotide , streptavidin , dna , chromatography , biochemistry , biotin , gene , chemistry

A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.

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