Analysis and Suppression of DNA Polymerase Pauses Associated with a Trinucleotide Consensus | Zendy

Daniel S. Mytelka | Zendy; Michael J. Chamberlin | Zendy

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Analysis and Suppression of DNA Polymerase Pauses Associated with a Trinucleotide Consensus

Author(s) -

Daniel S. Mytelka,

Michael J. Chamberlin

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.14.2774

Subject(s) - biology , dna polymerase , dna , genetics , dna clamp , polymerase , polymerase chain reaction , dna polymerase ii , dna sequencing , computational biology , gene , reverse transcriptase

We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist that make it difficult to incorporate nucleotides at these positions. We believe that these pauses result from constraints that make the conformation change involved in nucleotide selection more difficult. These pauses can obscure parts of DNA sequencing ladders and prevent DNA amplification by the polymerase chain reaction. The addition of betaine, and some related compounds, relieves these pauses.

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