A new efficient gene disruption cassette for repeated use in budding yeast | Zendy

Ulrich Güldener | Zendy

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A new efficient gene disruption cassette for repeated use in budding yeast

Author(s) -

Ulrich Güldener

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.13.2519

Subject(s) - biology , recombinase , cre recombinase , homologous recombination , locus (genetics) , genetics , gene , gene targeting , flp frt recombination , saccharomyces cerevisiae , expression cassette , marker gene , budding yeast , yeast , recombination , genetic recombination , recombinant dna , transgene , vector (molecular biology) , genetically modified mouse

The dominant kanr marker gene plays an important role in gene disruption experiments in budding yeast, as this marker can be used in a variety of yeast strains lacking the conventional yeast markers. We have developed a loxP-kanMX-loxP gene disruption cassette, which combines the advantages of the heterologous kanr marker with those from the Cre-lox P recombination system. This disruption cassette integrates with high efficiency via homologous integration at the correct genomic locus (routinely 70%). Upon expression of the Cre recombinase the kanMX module is excised by an efficient recombination between the loxP sites, leaving behind a single loxP site at the chromosomal locus. This system allows repeated use of the kanr marker gene and will be of great advantage for the functional analysis of gene families.

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