Kluyveromyces lactis killer system: analysis of cytoplasmic promoters of the linear plasmids | Zendy

Jörg Schickel | Zendy

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Kluyveromyces lactis killer system: analysis of cytoplasmic promoters of the linear plasmids

Author(s) -

Jörg Schickel

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.10.1879

Subject(s) - kluyveromyces lactis , biology , plasmid , kluyveromyces , promoter , gene , transcription (linguistics) , reporter gene , microbiology and biotechnology , cytoplasm , genetics , gene expression , saccharomyces cerevisiae , linguistics , philosophy

All of the 14 genes encoded by the cytoplasmic linear killer plasmids of Kluyveromyces lactis are preceded by upstream conserved sequences (UCSs), cis-acting elements involved in plasmid gene transcription. Using the bacterial glucose-dehydrogenase gene as a reporter, expression driven by seven cytoplasmic promoters was determined. The level of expression ranged from 0.5 to 6 nkat. The highest activity was displayed by UCS 6 of pGKL2 whereas the lowest level was obtained with UCS2 of pGKL2, all other values were in between. Sequences located 5' upstream the UCSs do not influence expression. As exemplified for UCS5 and UCS10, deletion led to an almost complete loss of expression.

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