Genotypic analysis of multiple loci in somatic cells by whole genome amplification | Zendy

Michael T. Barrett | Zendy; Brian J. Reid | Zendy; Geoffrey Joslyn | Zendy

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Genotypic analysis of multiple loci in somatic cells by whole genome amplification

Author(s) -

Michael T. Barrett,

Brian J. Reid,

Geoffrey Joslyn

Publication year - 1995

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/23.17.3488

Subject(s) - biology , ploidy , locus (genetics) , genetics , primer (cosmetics) , genome , somatic cell , dinucleotide repeat , microbiology and biotechnology , polymerase chain reaction , genotype , genetic marker , gene , allele frequency , chemistry , organic chemistry

To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Aliquots of these reactions were then used in locus-specific reactions using a single round of PCR cycles with individual sets of primers representing polymorphic markers for different regions. As a result, polymorphic markers for different chromosomal regions, including VNTRs and dinucleotide repeats, can be used to perform up to 30 locus-specific PCR assays with a single sample obtained from fewer than 1000 cells.

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