Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes | Zendy

Kai Tang | Zendy; Dong-Jing Fu | Zendy; Sven Kötter | Zendy; Robert J. Cotter | Zendy; Charles R. Cantor | Zendy; Hubert Köster | Zendy

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Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes

Author(s) -

Kai Tang,

Dong-Jing Fu,

Sven Kötter,

Robert J. Cotter,

Charles R. Cantor,

Hubert Köster

Publication year - 1995

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/23.16.3126

Subject(s) - duplex (building) , mass spectrometry , dna , desorption , matrix assisted laser desorption/ionization , denaturation (fissile materials) , biology , matrix (chemical analysis) , chromatography , nucleic acid denaturation , analytical chemistry (journal) , biochemistry , base sequence , chemistry , nuclear chemistry , organic chemistry , adsorption

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

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