Whole genome amplification of single cells: mathematical analysis of PEP and tagged PCR | Zendy

Fengzhu Sun | Zendy; Norman Arnheim | Zendy; Michael S. Waterman | Zendy

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Whole genome amplification of single cells: mathematical analysis of PEP and tagged PCR

Author(s) -

Fengzhu Sun,

Norman Arnheim,

Michael S. Waterman

Publication year - 1995

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/23.15.3034

Subject(s) - biology , processivity , primer (cosmetics) , genome , polymerase chain reaction , genetics , microbiology and biotechnology , computational biology , polymerase , dna , gene , physics , thermodynamics

We construct a mathematical model for two whole genome amplification strategies, primer extension preamplification (PEP) and tagged polymerase chain reaction (tagged PCR). An explicit formula for the expected target yield of PEP is obtained. The distribution of the target yield and the coverage properties of these two strategies are studied by simulations. From our studies we find that polymerase with high processivity may increase the efficiency of PEP and tagged PCR.

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