Open AccessAT-rich upstream sequence elements regulate spore germination-specific expression of theDictyostelium discoideum celAgene
Author(s) -
Rithwik Ramachandran,
John E. Blume,
Kalyan Ganguly,
Herbert L. Ennis
Publication year - 1995
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/23.15.3018
Subject(s) - biology , dictyostelium discoideum , mycetozoa , gene , dictyostelium , slime mold , sequence (biology) , gene expression , genetics , upstream (networking) , germination , spore germination , botany , computational biology , computer science , computer network
Two members of a family of spore germination-specific cDNAs, celA and celB, are expressed coordinately, exclusively during spore germination. In the present study the regulatory sequence elements responsible for celA germination-specific expression have been identified. The very AT-rich 81 bp sequence between -664 and -584 upstream of the translation initiation site was required for proper temporal transcription of the celA gene. This sequence is comprised of two cis elements, each of which was active by itself in allowing celA expression. Electrophoretic mobility shift assays showed that a factor(s) in an extract prepared from germinating spores bound to the celA regulatory region. One of the three complexes formed was specific for the germinating spore extract. The results are consistent with the notion that the factor(s) that binds to this regulatory region is involved in expression of celA.