A rapid and simple method to isolate and characterize highly polymorphic markers from the centromeric regions of the human chromosomes | Zendy

A. Laurent | Zendy; Bertrand Marçais | Zendy; Martine Muleris | Zendy; Gérard Roizès | Zendy

Open Access

A rapid and simple method to isolate and characterize highly polymorphic markers from the centromeric regions of the human chromosomes

Author(s) -

A. Laurent,

Bertrand Marçais,

Martine Muleris,

Gérard Roizès

Publication year - 1994

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/22.2.194

Subject(s) - biology , genetics , primer (cosmetics) , microsatellite , alu element , dna sequencing , gene mapping , chromosome , computational biology , microbiology and biotechnology , human genome , genome , dna , gene , allele , chemistry , organic chemistry

Using oligonucleotide primers complementary to the 3' ends of either the Alu or the L1Hs consensus sequences in conjunction with a primer complementary to alpha satellite subsets specific to different human chromosomes, it was possible to detect and characterize polymorphisms originating from the microsatellites which are often present downstream these repetitive elements. The methodology does not require cloning, sequencing or synthesis of specific primers. Centromeric location was confirmed by linkage analysis, in situ hybridization and sequencing. The method is proposed for the generation of polymorphic markers from all centromeric regions.

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