In vitro phosphorylation studies of a conserved region of the transcription factor ATF1 | Zendy

Norma Masson | Zendy; Joseph Abraham Christopher John | Zendy; K A Lee | Zendy

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In vitro phosphorylation studies of a conserved region of the transcription factor ATF1

Author(s) -

Norma Masson,

Joseph Abraham Christopher John,

K A Lee

Publication year - 1993

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/21.18.4166

Subject(s) - biology , phosphorylation , transcription factor , creb , serine , signal transduction , microbiology and biotechnology , dna , dna binding protein , transcription (linguistics) , conserved sequence , genetics , biochemistry , gene , linguistics , philosophy , base sequence

A large family of mammalian transcription factors including multiple variants of CREB, CREM and ATF1 have been implicated in signal transduction by cAMP and other cellular pathways. Although the roles of some members of the family have been characterised the function of ATF1 is poorly understood. We have identified one or more key serine residues that are required for a phosphorylation-induced conformational change in ATF1. The critical serines map to a putative transcriptional activation domain of ATF1 and affect the stability of ATF1 DNA-binding. Intriguingly phosphorylation is modulated by ATF1 homodimerization and by ATF1 binding to DNA. One of the key serine residues required for ATF1 phosphorylation is not conserved in CREB and CREM suggesting that it is likely to determine some specialised function of ATF1.

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