σs-dependent promoters inEscherichia coliare located in DNA regions with intrinsic curvature | Zendy

Manuel Espinosa- Urgel | Zendy; Antonio Tormo | Zendy

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σ^s-dependent promoters inEscherichia coliare located in DNA regions with intrinsic curvature

Author(s) -

Manuel Espinosa- Urgel,

Antonio Tormo

Publication year - 1993

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/21.16.3667

Subject(s) - promoter , biology , sigma , sigma factor , dna , escherichia coli , gel electrophoresis , microbiology and biotechnology , gene , genetics , gene expression , physics , quantum mechanics

Expression of a number of genes during stationary phase in Escherichia coli is controlled by the alternative sigma factor sigma s (KatF). Promoters recognized by sigma s do not present a well-defined consensus sequence in their -10 and -35 regions. By polyacrylamide gel electrophoresis of DNA fragments performed at different temperatures, and by computer prediction analyses, we have found that sigma s-regulated promoters are located in regions where DNA shows intrinsic curvatures. This feature does not appear in a stationary-phase-induced promoter which is not controlled by sigma s. We propose that DNA bending may help in recognition and/or binding of sigma s to stationary-phase-induced promoters.

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