Characterization ofChlorellavirus PBCV-1CviAII restriction and modification system | Zendy

Yanping Zhang | Zendy; Michael Nelson | Zendy; Joseph Nietfeldt | Zendy; Dwight E. Burbank | Zendy; James L. Van Etten | Zendy

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Characterization ofChlorellavirus PBCV-1CviAII restriction and modification system

Author(s) -

Yanping Zhang,

Michael Nelson,

Joseph Nietfeldt,

Dwight E. Burbank,

James L. Van Etten

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.20.5351

Subject(s) - biology , restriction enzyme , endonuclease , microbiology and biotechnology , gene , dna , restriction fragment , genetics , restriction map , dna methyltransferase , recognition sequence , methyltransferase , nucleic acid sequence , methylation

A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII, which cleaves after the G and does not cleave either CmATG or mCATG sequences, CviAII cleaves between the C and A and is unaffected by mCATG methylation. The M.CviAII and R.CviAII genes were cloned and their DNA sequences were determined. These genes are tandemly arranged head-to-tail such that the TAA termination codon of the M.CviAII methyltransferase gene overlaps the ATG translational start site of R.CviAII endonuclease. R.CviAII is the first chlorella virus site-specific endonuclease gene to be cloned and sequenced.

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