Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants | Zendy

Peter Schmieder | Zendy; Hans Ippel | Zendy; Hans van den Elst | Zendy; G. A. VAN DER MAREL | Zendy; Jacques H. van Boom | Zendy; C. Altona | Zendy; Horst Kessler | Zendy

Open Access

Heteronuclear NMR of DNA with the heteronucleus in natural abundance: facilitated assignment and extraction of coupling constants

Author(s) -

Peter Schmieder,

Hans Ippel,

Hans van den Elst,

G. A. VAN DER MAREL,

Jacques H. van Boom,

C. Altona,

Horst Kessler

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.18.4747

Subject(s) - heteronuclear molecule , coupling constant , proton , histone octamer , abundance (ecology) , biology , dna , carbon fibers , nuclear magnetic resonance , nuclear magnetic resonance spectroscopy , materials science , physics , biochemistry , nuclear physics , particle physics , nucleosome , composite number , composite material , histone , fishery

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a reasonable measuring time and offers valuable information for a detailed picture of DNA structure.

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