A new method for the isolation of recombinant baculovirus | Zendy

G. Patel | Zendy; Kim Nasmyth | Zendy; Nic Jones | Zendy

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A new method for the isolation of recombinant baculovirus

Author(s) -

G. Patel,

Kim Nasmyth,

Nic Jones

Publication year - 1992

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/20.1.97

Subject(s) - biology , recombinant dna , isolation (microbiology) , virology , computational biology , baculoviridae , genetics , microbiology and biotechnology , gene , spodoptera

An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectable marker. The viral DNA maintained its ability to replicate in insect cells. An efficient and rapid selection system was set up, to isolate viral recombinants in yeast; DNA from selected yeast colonies was transfected into insect cells to obtain recombinant virus. We demonstrate the utility of this system by isolating recombinant viruses that express two different members of the CREB/ATF family of transcription factors.

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