Cyclization of uridine monophosphate by diethyl pyrocarbonate | Zendy

F. Solymosy | Zendy; L. Ehrenberg | Zendy; I. Fedorcsák | Zendy

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Cyclization of uridine monophosphate by diethyl pyrocarbonate

Author(s) -

F. Solymosy,

L. Ehrenberg,

I. Fedorcsák

Publication year - 1975

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/2.6.985

Subject(s) - uridine , ribonuclease , biology , pancreatic ribonuclease , phosphate , hydrolysis , biochemistry , electrophoresis , chromatography , chemistry , rna , gene

Reaction between diethyl pyrocarbonate and uridine 2'-phosphate or uridine 3'-phosphate leads to the formation in high yields of uridine 2':3'-cyclic phosphate. This reaction product was identified in experiments involving (a) ultraviolet spectrophotometry, (b) paper chromatography, (c) high voltage paper electrophoresis at both pH 3.5 and 7.4, (d) acid hydrolysis, and (e) digestion with pancreatic ribonuclease.

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