Size fractionation of double-stranded DNA by precipitation with polyethylene glycol | Zendy

John T. Lis | Zendy; Robert Schleif | Zendy

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Size fractionation of double-stranded DNA by precipitation with polyethylene glycol

Author(s) -

John T. Lis,

Robert Schleif

Publication year - 1975

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/2.3.383

Subject(s) - polyethylene glycol , molecular mass , peg ratio , precipitation , fractionation , dna , divalent , chromatography , sodium , biology , biochemistry , chemistry , organic chemistry , meteorology , economics , enzyme , physics , finance

We show that DNA molecules of differing molecular mass are separable by selective precipitation with polyethylene glycol (PEG+.. Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA. Double-stranded DNA can be fractionated at least in the range of 3 times 10-7 to 1 times 10-5 daltons. The effects on PEG concentration, sodium chloride concentration, DNA concentration, pH, divalent ions, precipitation time, and centrifugal force have been determined. These studies show PEG precipitation offers a size fractionation method for DNA which is convenient, of high capacity, and applicable over a wide range of conditions. However, resolution is not high and separation of two species approaches 100% only if they differ in molecular mass by at least a factor of two.

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