Absence of mRNA for casein in free polysomes of lactating ewe mammary gland | Zendy

LouisMarie Houdebine | Zendy; P. Gaye | Zendy

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Absence of mRNA for casein in free polysomes of lactating ewe mammary gland

Author(s) -

LouisMarie Houdebine,

P. Gaye

Publication year - 1975

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/2.2.165

Subject(s) - polysome , biology , messenger rna , rna , casein , biochemistry , protein biosynthesis , microbiology and biotechnology , ribosome , gene

Total polysomal RNA or poly A- containing RNA extracted from free polysomes of lactating ewe mammary gland was unable to direct a significant synthesis of alpha-s- and beta-casein. Polyacrylamide gel electrophoresis of poly A- containing RNA revealed that the two major peaks of mRNA isolated from membrane-bound polysomes were not observed in mRNA isolated from free polysomes. Purified 125-I anti-alpha-s-casein could bind significantly to membrane-bound polysomes only. These data suggest that casein synthesis occurs almost exclusively on membrane-bound polysomes and that this specificity may be attributed to the presence of casein mRNAs on these polysomes and their absence from free polysomes.

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