Open AccessIn vitro synthesis of large peptide molecules using glucosylated single-stranded bacteriophage T4D DNA template
Author(s) -
Christian Hulen,
J Legault-Démare
Publication year - 1975
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/2.11.2037
Subject(s) - biology , dna , bacteriophage , in vitro , biochemistry , ribosome , dna synthesis , peptide , microbiology and biotechnology , combinatorial chemistry , escherichia coli , rna , gene , chemistry
Denatured Bacteriophage T4D DNA is able to stimulate aminoacid incorporation into TCA-precipitable material in an in vitro protein synthesis system according to base DNA sequences. Newly synthesized polypeptides remain associated with ribosomes and have a molecular weight in range of 15,000 to 45,000 Daltons.
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