Open AccessA TaqI polymorphism in the human cyclin A gene
Author(s) -
P. Pateriini,
Marco Di Mitri,
Cindy M. Martin,
Arnold Münnich,
C Bréchot
Publication year - 1991
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/19.9.2516
Subject(s) - taqi , library science , biology , classics , genetics , gene , polymorphism (computer science) , history , computer science , genotype
PCR Conditions: The PCR reaction is carried out in a total volume of 50 /*1 containing approximately 250 ng DNA, 2 units Taq DNA polymerase, 50 pmol of each primer, 200 /M dNTP's, 10 mM Tris-HCl pH 8.3, 50 mM KC1, 1.5 mM MgCl2, 0.001% gelatin. The amplification is performed for 30 cycles with an annealing temperature of 60°C. The amplified product is digested with TaqI and the DNA fragments are analyzed on 1.5% agarose gels.