A TaqI polymorphism in the human cyclin A gene | Zendy

P. Pateriini | Zendy; Maria Stella De Mitri | Zendy; Cindy M. Martin | Zendy; A Munnich | Zendy; Christian Bréchot | Zendy

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A TaqI polymorphism in the human cyclin A gene

Author(s) -

P. Pateriini,

Maria Stella De Mitri,

Cindy M. Martin,

A Munnich,

Christian Bréchot

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.9.2516

Subject(s) - taqi , library science , biology , classics , genetics , gene , polymorphism (computer science) , history , computer science , genotype

PCR Conditions: The PCR reaction is carried out in a total volume of 50 /*1 containing approximately 250 ng DNA, 2 units Taq DNA polymerase, 50 pmol of each primer, 200 /M dNTP's, 10 mM Tris-HCl pH 8.3, 50 mM KC1, 1.5 mM MgCl2, 0.001% gelatin. The amplification is performed for 30 cycles with an annealing temperature of 60°C. The amplified product is digested with TaqI and the DNA fragments are analyzed on 1.5% agarose gels.

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