NMR studies of a DNA containing 8-hydroxydeoxyguanosine | Zendy

Yasushi Oda | Zendy; Seichi Uesugi | Zendy; Morio Ikehara | Zendy; Susumu Nishimura | Zendy; Yasutoshi Kawase | Zendy; Hiroyuki Ishikawa | Zendy; Hideo Inoue | Zendy; Eiko Ohtsuka | Zendy

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NMR studies of a DNA containing 8-hydroxydeoxyguanosine

Author(s) -

Yasushi Oda,

Seichi Uesugi,

Morio Ikehara,

Susumu Nishimura,

Yasutoshi Kawase,

Hiroyuki Ishikawa,

Hideo Inoue,

Eiko Ohtsuka

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.7.1407

Subject(s) - biology , dna , genetics , computational biology

The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.

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