Nucleotide sequence of a cDNA encoding Wnt-1 of the Mexican axolotlAmbystoma mexicanum | Zendy

Ursula Busse | Zendy; J. Guay | Zendy; Charles Seguin | Zendy

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Nucleotide sequence of a cDNA encoding Wnt-1 of the Mexican axolotlAmbystoma mexicanum

Author(s) -

Ursula Busse,

J. Guay,

Charles Seguin

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.4.981

Subject(s) - ambystoma mexicanum , axolotl , biology , complementary dna , genetics , nucleic acid sequence , cdna library , evolutionary biology , gene , regeneration (biology)

Wnt-1 (int-1, wingless) is a proto-oncogene first identified in mammary gland tumors due to its inappropriate expression after activation by the mouse mammary tumor virus (1). A cDNA coding for the Mexican axolotl (Ambystoma mexicanum) Wnt-1 (AWnt-1) was found by screening a stage 18-embryo cDNA library with a 200 bp fragment of the axolotl Wnt-1 obtained by polymerase chain reaction (PCR) amplification. The axolotl Wnt-1 is rich in cysteine residues and has four possible sites for N-linked glycosylation (bold underlined). Comparison of the predicted amino acid sequences of the axolotl, mouse (2), Xenopus (3) and Drosophila (4) Wnt-1 proteins showed that A Wnt-1 is 78% identical to the mouse protein, 76% to Xenopus and 56% to Drosophila.

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