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The deoxyribonucleotide 5'CGCGTATATACGCG3' was synthesized and studied by NMR methods. This short, fully palindromic duplex also forms a hairpin under certain conditions described within. As such, it is considered to be a model for cruciform formation. We show that this sequence forms a four-membered loop and a duplex in solution. The duplex is shown to be a normal, B-DNA like helix, while the hairpin is shown to be a four-membered ATAT loop.

1H NMR of 5′CGCGTATATACGCG3′, a duplex and a four-membered loop

₁H NMR of 5′CGCGTATATACGCG3′, a duplex and a four-membered loop