Termination of transcription in an ‘in vitro’ system is dependent on a polyadenylation sequence | Zendy

Vicente J. Miralles | Zendy

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Termination of transcription in an ‘in vitro’ system is dependent on a polyadenylation sequence

Author(s) -

Vicente J. Miralles

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.13.3593

Subject(s) - polyadenylation , cleavage and polyadenylation specificity factor , cleavage factor , biology , transcription (linguistics) , cleavage stimulation factor , cleavage (geology) , post transcriptional modification , microbiology and biotechnology , promoter , transcription factor , rna , genetics , rna splicing , gene expression , gene , paleontology , linguistics , philosophy , fracture (geology)

Using HeLa cell nuclear extract as a source of the different transcription and polyadenylation factors and reverse transcription to analyze the levels of RNA 5' and 3' to the cleavage-polyadenylation site, an in vitro assay has been established to study polyadenylation coupled to transcription directed by different adenovirus promoters. The levels of transcription 5' and 3' to the cleavage site in the L3 polyadenylation region are practically the same as described previously, however, the level of transcription 3' to the cleavage site in the SV40 early polyadenylation region decreases immediately after the cleavage site indicating a termination of the transcription.

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