A rapid in vitro assay for HIV DNA integration | Zendy

Robert Craigie | Zendy; Kiyoshi Mizuuchi | Zendy; Frederic D. Bushman | Zendy; Alan Engelman | Zendy

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A rapid in vitro assay for HIV DNA integration

Author(s) -

Robert Craigie,

Kiyoshi Mizuuchi,

Frederic D. Bushman,

Alan Engelman

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.10.2729

Subject(s) - biology , dna , oligonucleotide , genome , rna , virology , dna replication , microbiology and biotechnology , genetics , computational biology , gene

Retroviruses synthesize a double stranded DNA copy of their RNA genome after infection of a permissive cell and subsequent integration of this DNA copy into the host genome is necessary for normal viral replication. Integration occurs by a specialized DNA recombination reaction, mediated by the viral IN protein. Because this reaction has no known cellular counterpart, it is a particularly attractive target in the search for specific inhibitors with low toxicity that may serve as therapeutic antiviral agents. We present a simple assay system that is suitable for screening potential inhibitors of HIV DNA integration. Only short oligonucleotides matching one end of HIV DNA and purified HIV IN protein are required as substrates. Furthermore, since each step of the assay can be carried out in the wells of microtiter plates, large numbers of reactions can be processed simultaneously.

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