2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event | Zendy

Matthew Cotten | Zendy; Berndt Oberhauser | Zendy; Helmut Brunar | Zendy; Armin Holzner | Zendy; Georg Issakides | Zendy; Christian R. Noe | Zendy; G. Schaffner | Zendy; Ernst Wagner | Zendy; Max L. Birnstiel | Zendy

Open Access

2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event

Author(s) -

Matthew Cotten,

Berndt Oberhauser,

Helmut Brunar,

Armin Holzner,

Georg Issakides,

Christian R. Noe,

G. Schaffner,

Ernst Wagner,

Max L. Birnstiel

Publication year - 1991

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/19.10.2629

Subject(s) - biology , rna , messenger rna , in vitro , snrnp , microbiology and biotechnology , antisense rna , biochemistry , rna splicing , gene

We describe the synthesis of 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2'-O-modified compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2'-O-methyl and 2'-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1-10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.

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