The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism | Zendy

Peter J. Bredenbeek | Zendy; Catherine J. Pachuk | Zendy; Ans F.H. Noten | Zendy; Jeroen Charité | Zendy; Willem Luytjes | Zendy; Susan R. Weiss | Zendy; Willy J. M. Spaan | Zendy

Open Access

The primary structure and expression of the second open reading frame of the polymerase gene of the coronavirus MHV-A59; a highly conserved polymerase is expressed by an efficient ribosomal frameshifting mechanism

Author(s) -

Peter J. Bredenbeek,

Catherine J. Pachuk,

Ans F.H. Noten,

Jeroen Charité,

Willem Luytjes,

Susan R. Weiss,

Willy J. M. Spaan

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.7.1825

Subject(s) - translational frameshift , biology , genetics , open reading frame , gene , nucleic acid sequence , polymerase , microbiology and biotechnology , peptide sequence , conserved sequence

Sequence analysis of a substantial part of the polymerase gene of the murine coronavirus MHV-A59 revealed the 3' end of an open reading frame (ORF1a) overlapping with a large ORF (ORF1b; 2733 amino acids) which covers the 3' half of the polymerase gene. The expression of ORF1b occurs by a ribosomal frameshifting mechanism since the ORF1a/ORF1b overlapping nucleotide sequence is capable of inducing ribosomal frameshifting in vitro as well as in vivo. A stem-loop structure and a pseudoknot are predicted in the nucleotide sequence involved in ribosomal frameshifting. Comparison of the predicted amino acid sequence of MHV ORF1b with the amino acid sequence deduced from the corresponding gene of the avian coronavirus IBV demonstrated that in contrast to the other viral genes this ORF is extremely conserved. Detailed analysis of the predicted amino acid sequence revealed sequence elements which are conserved in many DNA and RNA polymerases.

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