Capillary gel electrophoresis for rapid, high resolution DNA sequencing | Zendy

Harold Swerdlow | Zendy; Raymond F. Gesteland | Zendy

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Capillary gel electrophoresis for rapid, high resolution DNA sequencing

Author(s) -

Harold Swerdlow,

Raymond F. Gesteland

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.6.1415

Subject(s) - capillary electrophoresis , biology , gel electrophoresis , dna , chromatography , oligonucleotide , free flow electrophoresis , electrophoresis , dna sequencing , genomic dna , resolution (logic) , detection limit , microbiology and biotechnology , genetics , polyacrylamide gel electrophoresis , gel electrophoresis of proteins , biochemistry , chemistry , enzyme , artificial intelligence , computer science

Capillary gel electrophoresis has been demonstrated for the separation and detection of DNA sequencing samples. Enzymatic dideoxy nucleotide chain termination was employed, using fluorescently tagged oligonucleotide primers and laser based on-column detection (limit of detection is 6,000 molecules per peak). Capillary gel separations were shown to be three times faster, with better resolution (2.4 x), and higher separation efficiency (5.4 x) than a conventional automated slab gel DNA sequencing instrument. Agreement of measured values for velocity, resolution and separation efficiency with theory, predicts further improvements will result from increased electric field strengths (higher voltages and shorter capillaries). Advantages of capillary gel electrophoresis for automatic DNA sequencing instruments and for genomic sequencing are discussed.

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