Nucleotide sequence of the bovine enteric coronavirus BECV F15 mRNA 5 and mRNA 6 unique regions | Zendy

Nathalie Woloszyn | Zendy; Pascal Boireau | Zendy; Jacques Laporte | Zendy

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Nucleotide sequence of the bovine enteric coronavirus BECV F15 mRNA 5 and mRNA 6 unique regions

Author(s) -

Nathalie Woloszyn,

Pascal Boireau,

Jacques Laporte

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.5.1303

Subject(s) - biology , messenger rna , bovine coronavirus , nucleic acid sequence , genetics , nucleotide , coronavirus , sequence (biology) , microbiology and biotechnology , gene , virology , covid-19 , medicine , disease , pathology , infectious disease (medical specialty)

Bovine entenc coronavirus belongs to the monogeneric Coronavindae family; it induces very severe, often fatal diarrhoea in young calves. Described for the first time in the USA (1) an antigenically different strain F15 was isolated in France (2-3) The complete nucleotide sequence of the BECV F15 genome located between the S and M structural genes has been determined from 4 overlapping cDNA clones. This sequence is 962 nucleotide long. Two main open reading frames (ORF) corresponding to the unique regions of the subgenomic mRNA 5 and mRNA 6 were identified. ORF 5 of 327 nucleotides encodes a polypeptide of 12,807 daltons (NS2); ORF 6 of 252 nucleotides encodes a polypeptide of 9,585 daltons (NS23). NS2 arruno acid sequence is 53.2% homologous with 12,400 daltons (ORF 5a) coronavirus MHV-JHM polypeptide (4) and NS3 amino acid sequence is 61.5% homologous with the 10,200 daltons (ORF 5B) MHV-JHM polypeptide (4). The regions of homology involved in regulating the synthesis of the subgenomic mRNAs 5, 6 and 7 are underlined.

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