Localization and structural analysis of the ribosomal RNA operons ofRhodobacter sphaeroides | Zendy

Sylvia C. Dryden | Zendy; Samuel Kaplan | Zendy

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Localization and structural analysis of the ribosomal RNA operons ofRhodobacter sphaeroides

Author(s) -

Sylvia C. Dryden,

Samuel Kaplan

Publication year - 1990

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/18.24.7267

Subject(s) - biology , ribosomal rna , rhodobacter sphaeroides , genetics , 23s ribosomal rna , operon , transfer rna , ribosomal intergenic spacer analysis , internal transcribed spacer , 5s ribosomal rna , gene , rhodobacter , microbiology and biotechnology , rna , 18s ribosomal rna , ribosome , bacteria , escherichia coli , mutant

We have identified, cloned and sequenced the three ribosomal RNA (rRNA) operons (rrn) present in the facultative photoheterotroph Rhodobacter sphaeroides. DNA sequence analysis has identified the 16S, 23S, and 5S rRNAs, two tRNAs (ile and ala) in the spacer region between the 16S and 23S rRNAs, and an f-met tRNA immediately following the 5S rRNA gene of all three operons. Physical mapping, genetic analysis, and Southern hybridization data indicate that rrnA is contained on a large chromosome and rrnB and rrnC are contained on a second smaller chromosome. These findings are discussed in relation to the origins of diploidy.

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